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. 2019 Jun 18;116(26):12816–12821. doi: 10.1073/pnas.1904005116

Fig. 5.

Fig. 5.

Calcium induced opening of the PTP in permeabilized HAP1 cells. The calcium retention capacity of mitochondria was determined in digitonin permeabilized cells (2 × 107 cells per mL) in response to pulses of 10 μM CaCl2, in the absence and presence of 1 μM CsA. Mitochondrial uptake of extramitochondrial Ca2+ was monitored by the fluorescence of Calcium green-5N given in arbitrary units (a.u.). The collapse of the fluorescence signal corresponds to the opening of the PTP. (A) HAP1-WT cells. (B) HAP1-Δ(c+δ) cells. (C) Quantitation of the PTP displayed as the ratio of the number of calcium pulses required to induce the PTP in CsA inhibited (CRCi) and untreated (CRCo) samples (±SD). The experimental data for the number of calcium pulses required to induce the PTP are shown in SI Appendix, Table S3, and representative traces for HAP1-Δe, -Δf, -Δg, -ΔDAPIT, and -Δ6.8PL cells are given in SI Appendix, Fig. S5.