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. 2019 Jun 12;116(26):12958–12963. doi: 10.1073/pnas.1903896116

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Fig. 5. — LAPF locates on early endosomes and promotes aggregation of Cav1/2. (A) Confocal microscopy of the immunofluorescence staining of EEA1, RAB7, LAMP1, and overexpressed LAPF. (Magnification, 126×.) (B) Immunoblot analysis of anti-myc immunoprecipitated lysates of 293T cells cotransfected with V5-tagged LAPF and Flag-tagged Cav1. (C) Immunoblot analysis of anti-myc immunoprecipitated lysates of 293T cells cotransfected with V5-tagged LAPF and Flag-tagged Cav2. (D) Immunofluorescence analysis of overexpressed Cav1, Cav2, and LAPF in A549 cells. (Magnification, 252×.) Data are shown for one representative experiment from three independent experiments with similar results.