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. 2019 Apr 25;3(7):943–953. doi: 10.1002/hep4.1362

Figure 5.

Figure 5

miR‐30e inhibits SREBP‐1c and FASN in hepatocyte and replicon (genotype 2a) cells. (A) Huh7.5 cells were left uninfected (mock) or infected with HCV, and cell lysates were analyzed for SREBP‐1c and FASN expression by western blot using specific antibodies. The blots were reprobed with antibody to actin for protein load. (B) Huh7.5 cells were transfected with miR‐30e mimic or miRNA mimic negative control, and cell lysates were prepared after 48 hour post‐transfection. SREBP‐1c and FASN were detected by western blot analysis using specific antibodies. The blot was reprobed with antibody to actin for loading control. (C) SREBP‐1c and FASN were down‐regulated in Huh7.5 cells harboring the HCV genome‐length replicon cells. A densitometry scan is shown on the right in all panels. Statistical significance was analyzed using the two‐tailed Student t test; *P < 0.05, **P < 0.01.