Human PBMCs were treated with PBS or 100 ng/ml LPS at 37°C for 4 hours,
the expression of TF on PBMCs were analyzed by flow cytometry using PE-labeled
anti-TF antibody (A). Thrombin generation initiated by PBMCs was performed as
described in the Materials and Methods. PBMCs were treated with PBS, 100 ng/ml
LPS, or 100 ng/ml LPS plus 50 μg/ml anti-TF antibody (4501)
(n=3). Representative trace (B) and peak thrombin generation are shown
(C). **P < 0.01,
***P < 0.001,
t test.