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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Circ Genom Precis Med. 2019 May 6;12(6):e002390. doi: 10.1161/CIRCGEN.118.002390

Figure 7.

Figure 7.

Effect of PQBP1 silencing on cholesteryl ester (CE) accumulation in antiretroviral therapy (ART)-treated foam cells. THP-1 macrophages were first transfected with a pool of 3 PQBP1 siRNAs or a pool of 2 control siRNAs. Following a 24 hour pre-treatment with either DMSO (–), nelfinavir (15 μM), saquinavir (15μM), or ritonavir (30 μM), AcLDL (50 μg/mL) was added to the culture media for the following 48 hours. Drug treatment was maintained throughout the experiment. CE accumulation and PQBP1 expression levels were measured in cell lysates. Results are shown for 3 independent experiments with 3 technical replicates (n=9) for CE accumulation measurements and 2 technical replicates (n=6) for PQBP1 RNA level quantification. Data are expressed as mean±SEM. NELF, nelfinavir; SAQ, saquinavir; RIT, ritonavir.