KEY RESOURCE TABLE (uploaded separately)
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial Strains | ||
| E.coli BL21 (DE3) RIL expression strain | Agilent | Cat#230140 |
| E.coli BL21 (DE3) Gold expression strain | Agilent | Cat #230132 |
| Recombinant DNA | ||
| GFP-KNL-1 wt Hardened | Addgene | Id 45223 |
| pET-M30-MBP-TEV-KNL11–150 | This study | N/A |
| pET-M30-MBP-TEV-KNL11–80 | This study | N/A |
| pET-M30-MBP-TEV-KNL123–80 | This study | N/A |
| pET-M30-MBP-TEV-KNL122–80 | This study | N/A |
| pET-M30-MBP-TEV-KNL11–80S24A | This study | N/A |
| pET-M30-MBP-TEV-KNL11–80S24AS25A | This study | N/A |
| pET-M30-MBP-TEV-KNL11–80S60A | This study | N/A |
| pET-M30-MBP-TEV-KNL11–80SILK/AAAA | This study | N/A |
| pET-M30-MBP-TEV-KNL11–80S24DS25D | This study | N/A |
| pET-M30-MBP-TEV-KNL11–80S56D | This study | N/A |
| pET-M30-MBP-TEV-KNL11–80S60D | This study | N/A |
| pGEX-Aurora-INCENP or AUKB | M. Bollean Laboratory | Available on request |
| pRRP1B-Thio6-His6TEV-PP1 γ 7–323 | Addgene | Id 51770 |
| pRRP1B-Thio6-His6TEV PP1 α7–330 | Addgene | Id 51768 |
| pRRP1B-Thio6-His6TEV- PP1 α7–300 | Addgene | Id 26566 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tubulin protein ( 99% pure, Porcine brain) | Cytoskeleton, Inc. | Cat #T240 |
| General Tubulin Buffer | Cytoskeleton, Inc. | Cat # BST06 |
| Tubulin Glycerol Buffer | Cytoskeleton, Inc. | Cat # BST05 |
| GTP | Cytoskeleton, Inc. | Cat # BST06 |
| Paclitaxel | Cytoskeleton, Inc. | (Cat. # TXD01) |
| Gro 7 Chaperone | Takara clonetch | Cat #3340 |
| Critical Commercial Assays | ||
| Microtubule Binding protein Spin Down Assay Biochem Kit | Cytoskeleton, Inc. | Cat # BK029 |
| QuikChange site directed mutagenesis Kit | Agilent | Cat# 200524 |
| PDB search model | Kelker (2009) | PDBID: 3E7A |
| Deposited Data | ||
| BMRB (NMR assignment KNL-11–80 and pKNL-11–80 | This study | BMRBID: 27057, 27066 |
| PDB | This study | PDBID: 6CZO |
| Software and Algorithms | ||
| Topspin 3.1 and 3.2 | Bruker | https://www.bruker.com |
| UCSF SPARKY | Goddard et al., 2004 | https://www.cgl.ucsf.edu/home/sparky/ |
| NMR Pipe | Delaglio et al., 1995 | https://www.ibbr.umd.edu/nmrpipe/install.html |
| CARA | www.cara.nmr.ch | www.cara.nmr.ch |
| PHENIX | Zwart et al., 2008 | https://www.phenix-online.org/ |
| COOT | Emsley et al., 2010 | https://www.ccp4.ac.uk |
| Pymol | Schrodinger, LLC | https://www.pymol.org |
| NITPIC | Brautigam (2015) | http://biophysics.swmed.edu/MBR/software.html |
| SEDPHAT | Zhao et al. 2015 | http://biophysics.swmed.edu/MBR/software.html |
| GUSSI | Brautigam (2015) | http://www.biophysics.bioc.cam.ac.uk/?p=736 |
| XDS | Kabsch,W (2010) | http://homes.mpimf-heidelberg.mpg.de/~kabsch/xds |