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. 2019 Apr 17;25:35–49. doi: 10.1016/j.molmet.2019.04.008

Figure 2.

Figure 2

UCP1 dependent respiration is decreased in BAT of Sirt3KO mice. (A) UCP1 dependent respiration was measured in isolated mitochondria from iBAT of room temperature housed WT and Sirt3KO mice. 0.35 mg Mitochondria were energized with 25 μM PLC and UCP1 dependent respiration was determined as that which was inhibited by 2 mM GDP. N = 6/group. (B) UCP1 dependent respiration was measured in isolated mitochondria from iBAT of room temperature housed WT and Sirt3KO mice. 0.25 mg Mitochondria were energized with 5 mM G3P and UCP1 dependent respiration was determined as that which was inhibited by 2 mM GDP. N = 4/group. (C) UCP1 dependent respiration was measured in isolated mitochondria from iBAT of cold exposed WT and Sirt3KO mice. 0.25 mg Mitochondria were energized with 5 mM G3P and UCP1 dependent respiration was determined as that inhibited by 2 mM GDP. N = 4/group. (D) Western blotting analysis of UCP1 expression. N = 3/group. (E) Quantification of UCP1 western blotting. Data are represented as mean ± SEM. Student's t-test; Two-tailed and two-way ANOVA with Sidak's test were used, *p < 0.05 and **p < 0.01.