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. 2019 Jun 7;18(2):1212–1220. doi: 10.3892/etm.2019.7650

Figure 2.

Figure 2.

Inhibition of SNHG12 inhibits colorectal cancer cell proliferation and invasion. (A-D) SW620 and HT-29 cells were transfected with SNHG12 siRNA or NC siRNA, respectively. (A) RT-qPCR was used to examine the levels of SNHG12. CCK-8 assay using (B) SW620 and (C) HT-29 cells, and (D) a Transwell assay (magnification, ×200) were performed to assess cell proliferation and invasion, respectively. For A-D, **P<0.01 vs. NC siRNA. (E-H) Next, SW620 and HT-29 cells were transfected with SNHG12 expression plasmid or a blank vector, respectively. (E) RT-qPCR was used to determine the levels of SNHG12. CCK-8 assay using (F) SW620 and (G) HT-29 cells, and (H) a Transwell assay (magnification, ×200) were performed to study cell proliferation and invasion. For E-H, **P<0.01 vs. Blank. Groups: siNC, cells transfected with NC siRNA; siSNHG12, cells transfected with siRNA targeting SNHG12; SNHG12, cells transfected with SNHG12 overexpression vector; Blank, cells transfected with empty vector. SNHG12, small nucleolar RNA host gene 12; CKK-8, Cell Counting Kit 8; siRNA, small interfering RNA; NC, negative control; OD, optical density; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.