Figure 4.
SNHG12 inhibits miR-16 expression in CRC cells. (A) Silencing of SNHG12 increased the expression of miR-16 in SW620 and HT-29 cells. **P<0.01 vs. NC siRNA. (B) SW620 and HT-29 cells were transfected with SNHG12 expression plasmid or a blank vector, respectively. The expression of miR-16 was then examined using reverse transcription-quantitative polymerase chain reaction. **P<0.01 vs. Blank. Groups: siNC, cells transfected with NC siRNA; siSNHG12, cells transfected with siRNA targeting SNHG12; SNHG12, cells transfected with SNHG12 overexpression vector; Blank, cells transfected with empty vector. SNHG12, small nucleolar RNA host gene 12; siRNA, small interfering RNA; NC, negative control; miR, microRNA.
