Skip to main content
. 2019 Jun 13;18(2):1375–1383. doi: 10.3892/etm.2019.7669

Figure 1.

Figure 1.

Immunostaining of fibrotic biomarkers in esophageal tissue samples from mice. Immunohistochemistry was performed on esophageal tissue samples from mice in the chronic restraint stress and control groups. Immunostaining for (A) collagen type I, (B) TGF-β1, (C) SMAD-3 and (D) α-SMA fibrotic markers (magnification, ×200; scale bar, 50 µm). Quantification of (E) collagen type I, (F) TGF-β1, (G) SMAD-3 and (H) α-SMA positive areas. Data presented as the mean ± standard deviation (n=15). *P<0.001 vs. control. TGF-β1, transforming growth factor β1; SMAD-3, SMAD family member 3; α-SMA, α-smooth muscle actin.