Table I.

Primer sequences used in reverse transcription-quantitative PCR.

Gene	Primer sequence (5′-3′)	Size (bp)
Nox4	F: CACCTCTGCCTGCTCATTTGG	153
	R: AGTTGAGGTTCAGGACAGATGC
Nrf-2	F: CGAGATATACGCAGGAGAGGTA AGA	79
	R: GCTCGACAATGTTCTCCAGCTT
HO-1	F: CAGCCCCACCAAGTTCAAAC	101
	R: AGGCGGTCTTAGCCTCTTCTG
Collagen type I	F: GGAATGAAAGGGACACAGAGG	197
	R: TAGCACCATCATTTCCACGA
TGF-β1	F: GGACTCTCCACCTGCAAGAC	100
	R: GACTGGCGAGCCTTAGTTTG
SMAD-3	F: CATCGAGCCCCAGAGCAATA	88
	R: GTGGTTCATCTGGTGGTCACT
α-SMA	F: TGCTGACAGAGGCACCACTGAA	138
	R: CAGTTGTACGTCCAGAGGCATA
β-actin	F: TATTGGCAACGAGCGGTTC	75
	R: ATGCCACAGGATTCCATACCC

F, forward; R, reverse; Nox4, NAPDH oxidase 4; Nrf-2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase 1; TGF-β1, transforming growth-β1; α-SMA, α-smooth muscle actin.