Figure 5.

MACC1 can rescue effect of miR-338-3p in malignant melanoma. A375 cells were transfected with miR-338-3p mimic, miR-NC, MACC1, or Vector control. (A) Cell proliferation was measured by the MTT assay. The MTT assay was performed every 24 h for 3 days. (B) A wound-healing assay and (C) Transwell matrigel invasion assay were used to determine cell migration and invasion, respectively in A375 cells. Scale bar, 20 µm. Data are presented as the mean value ± standard deviation from triplicate experiments. **P<0.01, ***P<0.001 vs. miR-NC + Vector, ^#P<0.05, ^##P<0.01 vs. miR-338-3p + Vector; (D) The protein level of MACC1, PCNA, E-cadherin, N-cadherin and Vimentin was detected by western blot in A375 cells. OD, optical density; miR, microRNA; NC, negative control; PCNA, proliferating cell nuclear antigen; MACC1, metastasis-associated in colon cancer-1; E, epithelial; n, neural.