Figure 3.

(a) Protein abundance of endothelial nitric oxide synthase (eNOS) in endothelial cells exposed to sera from human subjects collected before (0 wk) and 1 h after (Acute) the first hot water immersion session and following heat therapy (8 wk). Data were normalized to a vinculin loading control and presented as mean±SE fold changes from 0 wk. Representative Western blot images are provided below. (b) Increases in endothelial tubule formation (with exposure to 8 wk vs. 0 wk serum) were significantly related to increases in eNOS.