Table 2.

Oligonucleotide primers used in this study.

Genes	Target/primer	Sequences (5′-3′)	Size (bp)	PCR conditions	Reference
16S rRNA gene	16S rRNA	F: TGCATTAGCTAGTTGGTG	356	Denaturation 95°C for 4 min, 30 cycles at 95°C 30s, 58°C 60s, 72°C 60s, and 72°C 10 min	[20]
		R: TTAAGAAACCGCCTGCGC
Species specific genes	E. faecalis	F: CACCTGAAGAAACAGGC	475	Denaturation 95°C for 4 min, 30 cycles at 95°C for 30 s, 55°C 60s, 72°C 60s, and 72°C for 7 min	[21]
		R: ATGGCTACTTCAATTTCACG
	E. faecium	F: GAGTAAATCACTGAACGA	1091
		R: CGCTGATGGTATCGATTCAT
	E. durans	F: TTATGTCCCWGTWTTGAAAAATCAA	295		[22]
		R: TGAATCATATTGGTATGCAGTCCG
	E. gallinarum	F: GGTATCAAGGAAACCTC	173
		R: CTTCCGCCATCATAGCT
	E. hirae	F: CTTTCTGATATGGATGCTGTC	187
		R: TAAATTCTTCCTTAAATGTTG
	E. mundtii	F: CAGACATGGATGCTATTCCATCT	98	Denaturation 95°C for 4 min, 30 cycles at 95°C for 30 s, 60°C 60s, 72°C 60s, and 72°C for 7 min
		R: GCCATGATTTTCCAGAAGAAT
	E. casseliflavus	F: TCCTGAATTAGGTGAAAAAAC	288	Denaturation 95°C for 4 min, 30 cycles at 95°C for 30 s, 55°C 60s, 72°C 60s, and 72°C for 7 min
		R: GCTAGTTTACCGTCTTTAACG
	E. avium	F: GCTGCGATTGAAAAATATCCG	368	Denaturation 95°C for 4 min, 30 cycles at 95°C for 30 s, 55°C 60s, 72°C 60s, and 72°C for 7 min
		R: AAGCCAATGATCGGTGTTTTT
Vancomycin resistance genes	vanA	F: GGGAAAACGACAATTGC	732	Denaturation 94°C 3 min, 30 cycles at 94°C 60 s, 54°C 60 s, 72°C 60 s, and 72°C 10 min	[21]
		R: GTACAATGCGGCCGTTA
	vanB	F: ACGGAATGGGAAGCCGA	647
		R: TGCACCCGATTTCGTTC
	vanC1/2	F: ATGGATTGGTAYTKGTAT	815/827
		R: TAGCGGGAGTGMCYMGTAA
Tetracycline resistance genes	msrA/B	F: GCAAATGGTGTAGGTAAGACAACT	400	Denaturation 95°C 180 s, 35 cycles at 93°C 30 s, 55°C 120 s, and 72°C 90 s	[13]
		R: ATCATGTGATGTAAACAAAAT
	mefA	F: AGTATCATTAATCACTAGTGC	348
		R: TTCTTCTGGTACTAAAAGTGG
	tetL	F: ATAAATTGTTTCGGGTCGGTAT	1107
		R: AACCAGCCAACTAATGACAATGAT
	tetK	F: TATTTTGGCTTTGTATTCTTTCAT	1159	Denaturation 95°C 60s, 35 cycles at 50°C 60 s, 72°C 30 s, and 72°C 300 s
		R: GCTATACCTGTTCCCTCTGATAA
	tetM	F: GCAGAATATACCATTCACATCGAAGT	700	Denaturation 95°C for 60s, followed by 40 cycles of 95°C for 15s, 60°C for 60s, and 72°C for 60s	[17]
		R: AAACCAATGGAAGCCCAGAA
Virulence genes	asa1	F: GCACGCTATTACGAACTATGA	375	Denaturation 95°C 3 min, 30 cycles at 95°C 30s, 56°C 30s, 72°C 60s, and 72°C 10 min	[23]
		R: TAAGAAAGAACATCACCACGA
	gelE	F: TATGACAATGCTTTTTGGGAT	213
		R: AGATGCACCCGAAATAATATA
	cylA	F: ACTCGGGGATTGATAGGC	688
		R: GCTGCTAAAGCTGCGCTT
	esp	F: AGATTTCATCTTTGATTCTTGG	510
		R: AATTGATTCTTTAGCATCTGG
	hyl	F: ACAGAAGAGCTGCAGGAAATG	278
		R: GACTGACGTCCAAGTTTCCAA