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. 2019 Jun 16;2019:3808432. doi: 10.1155/2019/3808432

Figure 2.

Figure 2

Eya2 regulates the proliferation and invasion of prostate cancer. (a) Western blot analysis of Eya2 protein in prostate cancer cell lines (LNCaP, PC-3, and DU145) and RWPE-1 (normal prostate cell line): there were higher levels of Eya2 protein in cancer cell lines than the normal cell line. Eya2 transfection significantly increased the levels of Eya2 protein and mRNA levels in PC-3 and LNCaP cells. The application of Eya2 shRNA significantly reduced the levels of Eya2 protein and mRNA levels in PC-3 and LNCaP cells. (b) Eya2 transfection led to an increase in proliferation rate in both PC-3 and LNCaP cell lines, while Eya2 shRNA knockdown reduced the proliferation rate. (c) Colony formation demonstrated that the overexpression of Eya2 upregulated colony number in both PC-3 and LNCaP cell lines, while Eya2 shRNA knockdown downregulated colony number. (d) Matrigel invasion assays demonstrated that the overexpression of Eya2 in both PC-3 and LNCaP cell lines increased the number of invading cells, while Eya2 shRNA knockdown decreased invading cell numbers. ∗ p<0.05.