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. 2019 Jul 1;33(13-14):844–856. doi: 10.1101/gad.325662.119

Figure 1.

Figure 1.

Daedalus is a mitochondrially localized protein involved in piRNA biogenesis. (A) Schematic representation of the CG10880/Daed domain structure and the two null alleles. Highlighted in red is the portion of coding sequence that is out of frame in the mutants. (B) Confocal images of GFP-CG10880 in ovaries (see also Supplemental Fig. S1E). Scale bar, 10 µm. (C) Fold changes in steady-state RNA levels of the indicated soma- and germline-specific transposons from ovaries. Values are relative to w1118 flies and normalized to rp49. (*) P < 0.05; (**) P < 0.001 (unpaired t-test). Error bars indicate standard deviation. n = 4. (D,E) Confocal images of Piwi, Armi, Aub, and Ago3 in ovaries (see also Supplemental Fig. S2C–E). (F) Size distribution of transposon-mapping small RNAs from ovaries. Sense reads are shown in blue, and antisense reads are in red. (G,H) Coverage plots of small RNA reads uniquely mapped to the dual-strand cluster 42AB (G) and the unistrand cluster flamenco (flam) (H). Shown are normalized reads per million (RPM). The mappability for an average 25-bp read length is shown at the bottom.