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. 2019 Jul 1;33(13-14):844–856. doi: 10.1101/gad.325662.119

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© 2019 Munafò et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Armi binds to piRNA precursors. (A) Scatter plot showing expression levels (RPM) of genes in Armi CLIP-seq (n = 4) against a mock immunoprecipitation (n = 3). piRNA clusters expressed in OSCs are highlighted in red, and selected protein-coding genes producing piRNAs are in blue. (B) Genome browser shot displaying Armi CLIP-seq and small RNA sequencing (sRNA-seq) reads uniquely mapping to the piRNA cluster flam (top panel) and a zoomed-in view of the first ∼50 kb (bottom panel). Shown are normalized RPM. The mappability tracks for 50- and 25-bp read lengths, respectively, are shown below. (C) Same as in B but showing the protein-coding gene tj. (D,E) Scatter plots showing expression levels (RPM) of antisense and sense transposon sequences in Armi CLIP-seq against a mock immunoprecipitation. Transposon sequences present in flam are highlighted in red, with dot size proportional to their abundance within flam according to dm6 Repeat Masker annotations.