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. 2019 Jul 1;33(13-14):741–746. doi: 10.1101/gad.326363.119

Figure 5.

Figure 5.

Expression of SNORD97 and SCARNA97 inhibits angiogenin cleavage of tRNAMet(CAT). (A) Arsenite-induced oxidative stress promotes processing of tRNAMet(CAT) into stable 3′ tRF in the absence of SNORD97 and/or SCARNA97. Human cells indicated above were incubated in either the presence or absence of 250 μM arsenite for 2 h before RNA extraction. Accumulation of 3′ tRF was monitored by Northern blotting. (B) Angiogenin mediates cleavage of tRNAMet (CAT). SNORD97 + SCARNA97 knockout cells either treated with arsenite or nontreated were further incubated for 2 h in a medium supplemented with 40 or 96 µM angionenin inhibitor N-65828 or 50 or 100 ng/mL recombinant angiogenin as indicated. RNAs were extracted from each cell line and analyzed by Northern blotting.