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. 2019 Jul 1;33(13-14):828–843. doi: 10.1101/gad.324814.119

Figure 4.

Figure 4.

E1AKD-induced YAP association with chromatin-bound TEADs causes chromatin remodeling and activation of MSC-specific enhancers. (A) Average ChIP-seq signal at 4 d after transfection with the indicated siRNAs centered at ATAC-seq peaks that increased more than fivefold following 4 d of E1AKD. (B) Heat map of H3K4me1 ChIP-seq reads from 4-d E1AKD cells at all ATAC-seq peaks that increased more than fivefold with E1AKD (new ATAC sites). Base pair span is ±3 kb from the center of the ATAC peak. (C) Heat maps of TEAD4 and H3K4me1 ChIP-seq reads at all new ATAC sites in HEK293 cells transfected with control siRNA for 4 d. Peaks were sorted by the amount of H3K4me1 signal in siCtrl HEK293 cells. Base pair span is ±3 kb from the center of the peak. (D) Protein levels or phosphorylation status of Hippo pathway proteins from 4-d siRNA transfected HEK293 cells. KU86 served as a loading control. (E) LATS1 protein levels from HEK293 cells transfected for 3 d with either siCtrl or siE1A and then treated with 5 µM MLN4924 or DMSO control for 8 h.