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. 2019 Jul 1;33(13-14):828–843. doi: 10.1101/gad.324814.119

Figure 5.

Figure 5.

YAP nuclear localization in E1AKD293 cells requires actin polymerization and sequestration of AMOTs. (A) Four-day E1AKD293 cells treated with either DMSO or LatB for 30 min were fixed and immunostained with anti-YAP (DH81X) and stained with phalloidin-iFluor and DAPI. (B) HEK293 cells were transfected with an empty vector plasmid or plasmid expressing RhoA(Q63L) for 3 d. Cells were fixed and stained as in A. (C) Confocal microscopy of 4-d E1AKD293 and control HEK293 cells fixed and immunostained with anti-AMOT and stained with phalloidin-iFluor and DAPI. (D) HEK293 cells were transfected with control siRNA or a mixture of siRNAs for AMOT and its two human paralogs, AMOTL1 and AMOTL2. Cells were fixed and stained as in A.