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. 2019 Jul 1;33(13-14):828–843. doi: 10.1101/gad.324814.119

Figure 6.

Figure 6.

YAP and TAZ are required for H3K27/18ac and redifferentiation of HEK293 cells when E1A is removed. (A) Box plots of expression (FPKM) of E1AKD-derepressed genes (more than twofold FPKM; q < 0.05) following 8 d of the indicated siRNA transfections. (E) E1A; (Y) YAP; (T) TAZ. (*) P < 1 × 10−7 as compared with siE1A. (B) Bar graphs of FPKM values of select genes that were defective for E1AKD-induced derepression when YAP and TAZ, YAP but not TAZ, or TAZ but not YAP were knocked down at the same time as E1A. (C) Total levels of H3K18ac and H3K27ac following the indicated siRNA transfections for 2 d. (YT) YAP and TAZ; (EYT) E1A, YAP, and TAZ. (D) Average ChIP-seq signal after transfection with the indicated siRNAs for 4 d centered at ATAC-seq peaks that increased more than fivefold. Base pair span is ±3 kb from the center of the peak. (E) Confocal microscopy of siRNA transfected HEK293 cells for 4 d fixed and stained for phalloidin-iFluor or DAPI. Scale bar, 20 µM.