Figure 2.

Depletion of splenic and liver macrophages in G-KO mice results in an increase in half-life and serum levels of IgG. G-KO mice were treated with clodronate (1.5 mg/dose) or PBS (control) liposomes at 0 h and 48 h. (a, b) ¹²⁵I-labeled mIgG1 was injected (i.v.) at 18 h, and whole body radioactivity levels determined at the indicated times (a; 3–4 mice/group) post-antibody injection. Data shown is representative of two independent experiments. (b) β-phase half-lives of mIgG1 were obtained by fitting the pharmacokinetic data to a decaying mono-exponential model. (c) Normalized IgG levels in serum samples collected 0 h prior to and 201 h post-first injection of clodronate liposomes. Error bars indicate SEM. Significant differences (**, p < .01; ***, p < .001; two-tailed Student‘s t-test) between the groups are indicated. N.S., no significant difference (p > .05; two-tailed Student‘s t-test). Data shown for panels b and c is combined from two independent experiments (5–9 mice/group).