Table 3.

Light chain variable region sequence variant filtering and counts.

	mAb
	S1	S2	F1	F2
Assembled reads	4,182,159		6,289,741
Germline matchesa	2,999	75,557	92,338		85,642
CDR3 length matches	2,216	61,507	91,211		84,825
Selected lineage markers	Q27R, T53S, W96	K30E, P96Q	S31D, L96		S31D, F96L
Lineage marker matches	142	5,023	36,210		18,521
Unique sequences	57	1,789	12,296		6,453
Minimum read count filterb	1	2	30		30
Unique CDR/Vernier, filteredc	28	72	63		40
Selected sequences	5	25	7		5

^aExcludes sequences that do not include entire variable domain upon assembly and with ambiguous residues.

^bNumber of reads necessary for variant sequence selection.

^cSequences with unique combination of residues in CDR and selected framework positions and read count numbers equal or greater than the minimum read count filter.