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. 2019 Jun 9;11(5):884–898. doi: 10.1080/19420862.2019.1618673

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 2. — Nuclease-directed integration of single copy antibody genes. HEK293 cells transfected with either plasmid αFGFR1-pD6 or αFGFR1-pD6 (50:50 mix) were introduced via TALE nuclease into the AAVS locus of HEK293 cells, selected for blasticidin resistance for 13 days and stained with either FGFR1-Dy633 (a) or FGFR2-Dy488 (b). Plots show single color histogram overlaying anti-FGFR1 and anti-FGFR2 transfected cells. Dot plot shows dual staining to detect binding to FGFR1 (y-axis) and FGFR2 (x-axis). Samples are: (c) 300 ng donor, CRISPR/Cas9 via Amaxa; (d) 100 ng donor, CRISPR/Cas9 via Amaxa; (e) 300 ng donor, TALEN via Amaxa; (f) 300 ng donor, TALEN via PEI transfection.