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. 2019 Jun 9;11(5):884–898. doi: 10.1080/19420862.2019.1618673

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 7. — Concentration dependent inhibition of PD-1 blockade in a cell-based assay. PD-L1 artificial antigen presenting CHO-K1 (aAPC/CHO-K1) cells were co-cultured with PD-1 effector Jurkat cells, which stably express human PD-1 and a NFAT-luciferase reporter, in the presence or absence of varying concentrations of anti-PD-1 antibodies. The luminescence signal (y-axis) is plotted against the concentration of inhibitory antibody for K5T8-9, K5E3-61, nivolumab, and the parental “wild-type” clone 331_1_C08.