Skip to main content
. 2019 Jun 9;11(5):884–898. doi: 10.1080/19420862.2019.1618673

Table 2.

Mammalian display affinity matured anti- PD-1 clones. The dissociation constant (KD), on rate (ka) and off rate (kd) were calculated from experimental data generated by a MASS2 SPR instrument (Sierra Sensors) as described in the materials and methods. The maximal response (Rmax) and a measure of the average deviation of the experimental data (χ2) is shown. The VH CDR3 window that was mutagenized is shown in the third column in single letter amino acid code for the original wild-type (WT) parental clone 337_1_C08. Each clone variant CDR3 window sequence is shown with variation from 337_1_C08 shown in single letter amino acid code and identity to parent with dot (.).

VH VL VH KD [nM] ka (Ms−1 x105) kd (s−1 x10−3) Rmax χ2 [RU2]
WT WT 
DRDYYDAG
 74 2.6 19 187 2.6
H5E2 WT 
.E..H...
 17 3.2 5.8 134 1.8
H5E2 2 
.E..H...
 30 2.2 6.6 111 1.2
H5E2 9 
.E..H...
 17 2.4 4.0 132 0.6
H5E2 23 
.E..H...
 54 2.4 12.8 175 1.0
H5A2 6 
.A..H...
 18 3.0 5.2 247 1.9
H5A2 9 
.A..H...
 24 2.1 5.1 137 0.8
H5W2 2 
.W..H...
 28 2.0 5.7 175 1.9
H5T8 82 
...H..T
 14 1.8 2.5 126 0.4
H5W8 WT 
...H..W
 64 0.9 5.5 75 3.4
K5T7 WT 
...K.T.
 16 3.8 6.1 121 0.8
K5T7 12 
...K.T.
 14 3.2 4.5 127 0.3
K5T8 WT 
...K..T
 16 2.8 4.6 164 2.8
K5T8 2 
...K..T
 10 2.2 2.3 179 0.3
K5T8 6 
...K..T
 9 4.4 4.1 114 1.1
K5T8 9 
...K..T
 2.9 2.6 0.8 155 2.7
K5S8 3 
...K..S
 21 3.1 6.5 52 0.4
K5D2 2 
.D..K...
 22 2.9 6.6 136 0.3
K5E3 61 
..E.K...
 4.8 3.7 1.8 107 1.5
K5L2 12 
.L..K...
 24 2.8 6.8 200 2.5
R5V8 9 
....R..V
 3.9 5.2 2.1 84 1.3
R5V8 2 
....R..V
 35 1.8 6.3 209 2.3
Q5W2 25 
.W..Q...
 7.7 3.0 2.3 188 0.8
A5M7 13 
....A.M.
 14 1.3 1.8 60 0.4
WT 9 
........
 18 2.1 3.7 139 1.0
L3N7 WT 
..L...N.
 27 2.1 5.7 204 0.8
H4K4 123 
...H...K
 10 2.7 2.8 62 0.5
I2 34 
.I......
 5.4 4.1 2.2 80 1.5
I2I8 83 
.I....I.
 6.4 3.1 2.1 49 1.6
K5T8 86 
....K..T
 7.6 4.3 3.1 71 1.2
Nivolumab     2.8 5.0 1.2 28 0.1