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. 2019 Jun 4;11(5):965–976. doi: 10.1080/19420862.2019.1612690

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 5. — Comparison of the novel GS mutants to GSwt as a selection marker in generating stable antibody-producing cell lines. (a) Comparison of the antibody GA101 titer production in CHO-GS^−/- clone 1 stable pools generated using GSwt, GSwt + 25 µM MSX, and the various GS mutants (as labeled) as the selection condition. The antibody GA101 was expressed in a tricistronic vector configuration. (b) Stability assessment of antibody titer from single clones isolated from one of the GSwt + MSX stable pools over at least 60 generations. Titers were normalized to week 0 (MSX removal) for each clone in L-glutamine-free media. (c) Stability assessment of antibody titer from single clones isolated from one of the GS (D63A) stable pools over at least 60 generations. Titers were normalized to week 0 for each clone in L-glutamine-free media. (d) Stability assessment of antibody titer from single clones isolated from one of the GS (S257A) stable pools over at least 60 generations. Titers were normalized to week 0 for each clone in L-glutamine-free media.