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. 2019 Jun 4;11(5):965–976. doi: 10.1080/19420862.2019.1612690

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 6. — Growth and productivity assessment of single clones derived from GSwt + MSX and GS (R324C) selection in batch culture analysis. The stable pools were generated from CHO-GS^−/- clone 2. Growth curves of 4 and 5 randomly selected single clones isolated from GSwt + MSX (a) and R324C (b) stable pools, respectively, are shown. Viable cell density (VCD) was measured every day. The antibody GA101 titer of clones derived from GSwt + MSX (c) and R324C (d) stable pools were measured using nephelometer every other day. Growth and productivity were assessed until the viability dropped below 50%. Antibody GA101 titer measurement of top 20 producing clones from GSwt + MSX (e) and R324C (f) stable pools were assessed in a 14-day batch culture. Stability assessment was done for the single clones with productivity more than 150 mg/L of the antibody isolated from GSwt + MSX (g) and R324C (h) stable pools. The single clones were seeded at 3x10³/ml in glutamine-free 50/50 medium for a 14-day batch culture analysis of antibody GA101 production stability at week 0, 4, 8, and 12. Titers were analyzed via nephelometer.