Figure 3.

Binding of different Db3-43xhu225-Ig molecules to EGFR and HER3 by ELISA. A + B Binding of Db3-43xhu225-Ig molecules to immobilized EGFR-His (a) or HER3-His (b). Parental antibodies IgG hu225 and IgG 3-43 were included as a control. Bound antibodies were detected with an HRP-conjugated anti-human Fc secondary antibody. Mean ± SD, n = 3. (c) Simultaneous binding of both antigens was analyzed using EGFR-Fc as immobilized antigen. Serial dilution of different antibodies was added to the wells. Finally, the second antigen, HER3-His, was added to the wells and bound HER3-His was detected using an HRP-conjugated anti-His secondary antibody. Mean ± SD, n = 3.