Table 1.

EC₅₀ values [pM] of bispecific Db3–43xhu225-Ig molecules determined by ELISA binding experiments or flow cytometry analysis. Mean ± SD, n = 3.

	ELISA [pM]				Flow cytometry [pM]
		EGFR-His	HER3-His	EGFR-Fc + Her3-His	FaDu
Db3–43xhu225-CH1/CL-Fc		92 ± 19	150 ± 19	163 ± 44	140 ± 6
Db3-43xhu225-EHD2-Fc		98 ± 16	151 ± 19	160 ± 26	180 ± 2
Db3-43xhu225-hetEHD2-Fc		118 ± 34	173 ± 42	204 ± 27	238 ± 9
Db3-43xhu225-MHD2-Fc		100 ± 23	140 ± 34	169 ± 18	206 ± 19
IgG hu225		111 ± 41	-	-	129 ± 5
IgG 3-43		-	148 ± 48	-	8 ± 1