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. 2019 May 8;11(5):826–836. doi: 10.1080/19420862.2019.1608143

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© 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 2. — Preparation of anti-CD20 mAbs with core-fucosylated homogeneous N-glycans. This illustration details the preparation of anti-CD20 mAbs having two N-glycan chains with G2F structure. (1) digestion with wild-type Endo S and Endo D for truncating N-glycans, (2) transglycosylation with Endo F3 mutant (D165Q) in the presence of oxazolinated glycans as a glycan donor, (3) the separation and purification of anti-CD20 mAbs with two core-fucosylated N-glycan chains using cation-exchange column chromatography. Yellow circle, Gal; green circle, Man; blue square, N-acetylglucosamine; red triangle, fucose.