Figure 6.

MiR‐4286 reduces PTEN expression by directly targeting its 3′‐UTR. (A) Schematic diagram suggesting the subcloning of the miR‐4286 binding site we predicted at position 2969‐2975 of the PTEN 3′‐UTR into a psiCHECK‐2 luciferase construct， as well as predicted duplex formation consisting of binding sites of miR‐4286， miR‐4286 wild‐type, or miR‐4286 mutant. (B, C) Luciferase activity of the formation including the wild‐type or mutant PTEN 3′‐UTR reporter gene in A549 and H226 cells co‐transfected with the negative control (NC) or miR‐4286. Scrambled sequences were assigned to the NC. Relative Renilla luciferase activity was determined and normalized against the firefly luciferase activity