Skip to main content
. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Methods Enzymol. 2018 Aug 31;612:443–465. doi: 10.1016/bs.mie.2018.07.008

Table II.

Oligos for library prep generation

o199-P 5’ AUGUACACGGAGUCGACCCGCAACGCGA/3phos/3’
Linker-1 (NEB) 5’ App/CTGTAGGCACCATCAAT/3ddC 3’
o225-linker-mini-indexed 5’ /5Phos/AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT/iSp18/CACTCA/iSp18/CAAGCAGAAGACGGCATACGAGATATTGATGGTGCCTACAG 3’
JS-ind1 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGACACTCTTTCCCTACACGACG 3’
JS-ind2 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTACACTCTTTCCCTACACGACG 3’
JS-ind3 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCACACTCTTTCCCTACACGACG 3’
JS-ind4 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAACACTCTTTCCCTACACGACG 3’
JS-ind5 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACACTCTTTCCCTACACGACG 3’
JS-ind6 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACACTCTTTCCCTACACGACG 3’
JS-ind7 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACACTCTTTCCCTACACGACG 3’
JS-ind8 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAACACTCTTTCCCTACACGACG 3’
JS-ind9 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACACTCTTTCCCTACACGACG 3’
JS-ind10 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTACACTCTTTCCCTACACGACG 3’
JS-ind11 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACACACTCTTTCCCTACACGACG 3’
JS-ind12 5’AATGATACGGCGACCACCGAGATCTACACGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACACTCTTTCCCTACACGACG 3’
oJS231 5’ CAAGCAGAAGACGGCATACGAGATATTGATGGTGCC 3’