Figure 3.

Embryonic rescue of DINE expression is sufficient for KO;Tg^WT mice to survive and develop normally. A, The appearance of WT, KO, KO;Tg^WT, and KO;Tg^mut mice at P0. WT and KO;Tg^WT mice were viable, whereas KO and KO;Tg^mut mice exhibited cyanosis and died immediately after birth. B, The table shows the number of littermates from each genotype at E18.5 and P0, as well as the proportion of surviving pups to total pups when crossing with mice carrying the DINE^WT or DINE^mut transgene, respectively. Before birth, all genotypes were observed at a Mendelian ratio, but KO and KO;Tg^mut pups died immediately after birth. C, Kaplan–Meier survival curve of WT, KO, KO;Tg^WT, and KO;Tg^mut mice from E18.5 to 8 weeks were analyzed by log-rank tests (p < 0.001). D, The immunohistochemical staining of DINE in spinal motor neurons of WT and KO;Tg^WT at P8. E, NMJs in the diaphragm from adult WT and KO;Tg^WT mice were immunostained using antibodies specific for PGP9.5 (green) and BTX (red). Scale bars: D, 200 μm; E, 50 μm.