Figure 9.

Conditioned media derived from MPP⁺-treated neuronal cultures induce an increase in LCN2 expression in glial cultures. A, Diagram of the experimental design. B, SH-SY5Y neurons differentiated by treatment with 10 μm RA for 5 d were exposed to 100 μm MPP⁺ and the levels of LCN2 expression in the culture medium were measured by ELISA at 24 h after the MPP⁺ treatment (t test; n = 3). C, Mixed glial cultures including astrocytes and microglia were treated with CM derived from untreated or MPP⁺-treated neuronal cultures and the levels of LCN2 protein in the culture medium were measured by ELISA at 24 h after the CM treatment. **p < 0.01, significantly different from the untreated CM control (t test; n = 3). D, E, Increased levels of LCN2 mRNA and protein in the mixed glia were confirmed by traditional RT-PCR (D) and Western blotting (E), respectively, 24 h after the CM treatment.