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. 2016 Apr 20;36(16):4534–4548. doi: 10.1523/JNEUROSCI.1715-15.2016

Figure 2.

Figure 2.

Neuritin promotes axonal branching of hippocampal granule neurons in organotypic slice cultures. A, Images and reconstructions of axons and dendrites of a granule neuron expressing EGFP or expressing EGFP and neuritin in hippocampal slice culture. Slices were fixed and imaged at DIV 11. Scale bar, 50 μm. B, Quantification of axonal length (p = 0.963), the number of primary branches per 100 μm of axon (p = 0.041), and the average length of primary branches (p = 0.005); n = 18 total for each, and the data were collected from three independent experiments. *p < 0.05 (one-way ANOVA). ***p < 0.005 (one-way ANOVA). C, Reconstructions of axons and dendrites of granule neurons expressing EGFP in hippocampal slice cultures treated with 5 μm of KA or the vehicle (PBS). Scale bar, 50 μm. D, Quantification of axonal length (p = 0.648), the number of primary branches per 100 μm of axon (p = 0.047), and the average length of primary branches (p = 0.007); n = 18 for each, and the data were collected from three independent experiments. *p < 0.05 (one-way ANOVA). ***p < 0.005 (one-way ANOVA).