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. 2016 May 25;36(21):5785–5798. doi: 10.1523/JNEUROSCI.3986-15.2016

Figure 3.

Figure 3.

Altered phosphorylation drives tau accumulation and oligomerization in glaucomatous retinas. A, Pearson correlation analysis of 100 kDa/total tau vs total tau demonstrated a positive association between the levels of the 50 kDa species and the 100 kDa/50 kDa ratio. B, No significant correlation was observed between the levels of the 55 kDa species and the 100 kDa/55 kDa ratio. C, D, Analysis of phosphorylated tau/total tau vs total tau showed that phosphorylation of the PHF1 epitope (S396, S404) correlated positively with 50 kDa tau accumulation, while no significant correlation was found for 55 kDa tau. E, F, A negative correlation was found between S199 phosphorylation on 50 and 55 kDa tau and their levels. R, correlation coefficient. N = 9/group. G, H, Western blot analysis of retinal extracts in nondenaturing conditions using T22, an antibody that recognizes tau oligomers, confirmed the appearance of tau oligomeric complexes in glaucomatous retinas (OHT, N = 5; Intact, N = 3; Student's t test, *p < 0.05, ***p < 0.001). Vertical lines represent nonconsecutive samples from the same gel.