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. 2016 May 25;36(21):5775–5784. doi: 10.1523/JNEUROSCI.3250-15.2016

Figure 3.

Figure 3.

Characterization of the Sox11-shRNA-expression vector. A, Experimental procedure. pCAG-mCherry (0.3 mg/ml) and pSUPER-shSox11 (1.65 mg/ml) were cotransfected into layer 2/3 neurons using in utero electroporation at E15.5, and coronal sections were prepared at E18.5 and stained with anti-Sox11 antibody and Hoechst 33342. B, Confocal microscopic images of cells transfected with either control (arrow) or shSox11 (arrowhead) vectors. mCherry-positive transfected neurons in the intermediate zone are shown. Scale bar, 25 μm.