Table 3.

Effects of systemic application of AAV9–Kir4.2–EGFP

Line		HD noninjected		HD injected		p	Test	% Change	Meaning n
		Mean ± SE	n	Mean ± SE	n
	Animals
1	Age at death (d)	362.9 ± 5.5	9	349.6 ± 2.9	10	NS	tt		Animals
2	Final body weight (g)	19.64 ± 0.33	9	20.22 ± 0.39	10	NS	tt		Animals
3	Number of CAG repeats	188.1 ± 1.8	9	186.6 ± 1.6	10	NS	tt		Animals
	Transduction yield (3 weeks)
	Motor cortex
4	#S100β with Kir4.1-EGFP/#S100β with GFAP (%)			87.7 ± 13.6	27				VFs
	Striatum
5	#S100β with Kir4.1-EGFP/#S100β with GFAP (%)			35.5 ± 4.7	45				VFs
	Astrocyte physiology
	Basic properties
6	Membrane potential at break in with GluR blockers (mV)	−75.8 ± 0.6	122	−79.3 ± 0.4	38	<0.01	MWT	+5	Cells
7	Ba-induced changed of Rm (% CTRL)	43.6 ± 9.2	17	76.4 ± 12.6	13	<0.05	tt	+75	Cells
	Kir4.1-related VAC and GTCs in response to [Rubi-Glu] 3 mm
8	Ba-sensitive VAC at Vh of 100 mV (pA)	793 ± 92	26	1388 ± 130	15	<0.001	tt	+75	Cells
9	GTC amplitude at Vh of −120 mV in CTRL (pA)	543 ± 20	30	447 ± 22	33	<0.05	tt	−18	Cells
10	GTC amplitude at Vh of −120 mV in Ba (pA)	960 ± 61	19	732 ± 46	19	<0.01	tt	−24	Cells
	Neuron physiology
	Basic properties of SPNs
11	Membrane potential at break in (mV)	−73.1 ± 0.5	17	−74.2 ± 1.2	21	NS			Cells
12	Input resistance (MΩ)	232.6 ± 13.2	17	269.5 ± 21.8	21	NS			Cells
13	Rheobase (pA)	8.8 ± 0.5	11	7.25 ± 0.4	12	NS			Cells
14	Maximal firing frequency (action potentials/s)	34.8 ± 1.6	11	36.5 ± 3.1	12	NS			Cells
	Corticostriatal input to SPNs
15	Decay time constant of EPSCs	6.43 ± 0.31	4	4.63 ± 0.48	4	<0.05	MWT	−28	Cells
	Spontaneous activity
16	Average frequency of action potential-dependent (TTX-sensitive) events (s)	1.85 ± 0.47	15	0.63 ± 0.10	21	<0.05	MWT	−66	Cells

MWT, Mann–Whitney U test; tt, t test.