Fig. 4.

—A strategy to assess predictions of binding change through the lens of enhancer activity. (A) Change in regulatory activity between orthologous enhancers is estimated from difference between output scores of activity classifiers that use Drosophila melanogaster and Drosophila virilis ChIP profiles, respectively, as input. (B) An alternative estimate of change in regulatory activity between orthologous enhancers, similar to strategy in (A), except that D. virilis activity classifier uses “imputed” D. virilis ChIP profiles as input. Imputation of D. virilis ChIP scores is based on D. melanogaster ChIP scores and ΔChIP scores predicted from motif- and/or accessibility-level interspecies changes.