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. 2016 Sep 7;36(36):9454–9471. doi: 10.1523/JNEUROSCI.0936-16.2016

Figure 9.

Figure 9.

A–J, Pten regulates differentiation of all amacrine cell subtypes. Immunolabeling of P7 wild-type and Pten cKO retinas with antibodies against AP2α (A, A′), Pax6 (C–C‴, green), Barhl2 (C–C‴, red), GAD65 (E, E′), GlyT1 (G, G′), and Neurod6 (I, I′). Barhl2 and Pax6 are colabeled, as ectopic Barhl2 expression is seen in Pten cKO retinas (C′). Blue is a DAPI counterstain. Arrowheads in E,E′,G,G′ mark amacrine cell bodies in the INL. The graphs show the number of AP2α+ (B), Barhl2+Pax6+ (D), GAD65+ (F), GlyT1+ (H), and Neurod6+ (J) cells per field in P7 wild-type and Pten cKO retinas. *p < 0.05; **p < 0.01; ***p < 0.001.