Figure 5.

Characteristics of the generated HCs. A, There are no EdU⁺Myo6⁺ or EdU⁺Prestin⁺ HCs in the cochleae of control mice. B, In the cochleae of β-cat-OE/Notch1-KO/Atoh1-OE mice, the EdU⁺Myo7⁺ HCs could be labeled with Myo6, but only very few mitotic HCs in the sensory region could be stained with Prestin. Scale bar, 50 μm. C–E, The bundle structures of the new generated HC in the cochleae of control and β-cat-OE/Notch1-KO/Atoh1-OE mice. Arrows indicate the EdU⁺Myo7⁺ HCs. F, G, For showing the expression of transgene, the HA tag was labeled in the cochleae of Sox2^CreER/T2/Atoh1-OE mice. H, Quantification of the EdU⁺Myo7⁺ and EdU⁻Myo7⁺ HCs in GER of β-cat-OE/Notch1-KO/Atoh1-OE mice. Data in H are presented as mean ± SEM. I, J, Quantification of Sox2⁺ EdU⁺ SCs in the sensory region and GER. Data in F and G are presented as mean ± SEM. *p < 0.01. Scale bars: A3, C1, I1, 50 μm; C3, 10 μm.