Figure 3.

Differential recruitment of Te2 versus Te1 cortex at the late time interval of the consolidation of recent fearful memories. A, Schematic design of Te1 or Te2 inactivation performed at 4 d after training. B, C, Blockade of Te2 alone obtained by administering TTX or muscimol into the Te2 impaired the subsequent fear memory recall, measured as freezing to the overall CS presentation (B) and to the total time spent freezing during CS presentation and intertrial intervals (C). D, E, Conversely, at the same time point, Te1 inactivation did not affect fear memories. F, Experimental design of Te2 blockade performed 15–20 min after CS–US pairing. G, Te2 inactivation performed shortly after training did not affect recent fear memories. Fear response measured as a percentage of total immobility (freezing) both at 2 min before (pre-CS) and during the overall CS presentation (CS) in saline- and TTX-injected rats. H, Similar results were observed by measuring overall freezing responses during CS presentation and intertrial time intervals. All values are reported as mean ± SEM.