Figure 11.

Cyp1b1 expression is downregulated in glioma vessels, going along with decreased RA target gene expression. A, Representative pictures of H&E staining of control brain hemisphere (left) and tumor-bearing hemisphere (right) of mouse brains after intracranial tumor transplantation. Scale bar, 1 mm. B, Corresponding IF staining for ColIV and Podxl. Scale bar, 100 μm. C, qRT-PCR analysis for Axin2, Cyp1b1, Ahr, as well as RA target genes and Ang2 in the tumor endothelium compared with ECs from the contralateral hemisphere (dashed line). Data represent means of three independent experiments. *p < 0.05. **p < 0.01. ***p < 0.001. Error bars indicate SEM.