Figure 4.

Noncanonical Wnt signaling is not able to increase, but rather decreases Cyp1b1 expression in ECs. A, qRT-PCR for Axin2 and Cyp1b1 in pMBMECs stimulated with Wnt5a CM for 5 d relative to control CM (n = 3). B, Western blot for JNK and p-JNK as well as NFAT1 in β-catenin WT and β-catenin KO ECs. NFAT1 represents both the phosphorylated, inactive (135 kDa) as well as the dephosphorylated, active (120 kDa) protein. LaminB1 served as loading control. Quantification shows normalized signal intensity of p-JNK relative to JNK as well as of active NFAT. Signal intensity in β-catenin KO ECs are shown relative to β-catenin WT ECs (dashed line). *p < 0.05. **p < 0.01. ***p < 0.001. ns, Not significant. C, qRT-PCR for Cyp1b1 and Cyp1a1 in β-catenin WT ECs treated with EtOH (Contr.), 1 μm CsA, or 20 μm SP600125 for 1 h (n = 2). Error bars indicate SEM.