Figure 9.

20-HETE modulates junction protein expression. Quantification and representative Western blots for VE-cadherin (Cdh5) (A) and occludin (Ocln) (B) in mouse brain ECs after treatment with 10, 50, and 100 nm 20-HETE for 24 h. Protein levels after 20-HETE treatment were normalized to protein levels of control condition, respectively (dashed lines). α-Tubulin is shown as loading control. Data represent means of four or five independent experiments. C, Western blots and quantification for caveolin in mouse brain ECs after treatment with 10 nm 20-HETE for 24 h (n = 3). *p < 0.05. **p < 0.01. ***p < 0.001. ns, Not significant. Error bars indicate SEM.