Figure 6.

Disulfiram and diphenhydramine hydrochloride prevent the full development of EAE. A, EAE scores of mice administrated with disulfiram or diphenhydramine (10 mg/kg) by intraperitoneal injection daily after immunization. Data were analyzed using nonparametric Mann–Whitney U test (n = 10 mice per group). B, C, Representative spinal cord sections of H&E staining (B) and LFB staining (C) from EAE mice at day 15 after immunization. Arrows in B indicate inflammatory infiltration (n = 4 mice per group). Scale bars, 80 μm. D, E, Quantification of spinal cord infiltration in WM (D) and the percentage of demyelinated WM in total WM (E) in B and C. F, G, qPCR analysis of miR-30a (F) and IRF4 (G) in CD4⁺ T cells from mice administrated with disulfiram or diphenhydramine for 15 d (n = 3 mice; *p < 0.05, **p < 0.01, ***p < 0.001 vs control; A, Mann–Whitney U test; D–G, one-way ANOVA with Dunnett's post hoc test). Data are from at least three experiments and are shown as mean ± SEM.