Figure 1.

rsu-1 encodes a conserved LRR protein involved in L-AChR distribution. A, Structure of the rsu-1 locus. Black boxes, Coding regions; vertical black line, point mutation; dashed rectangle, details of the point mutation; bracket, full deletion of the locus. B, Left column, Stitched confocal projections of the anterior region of wild-type and rsu-1(kr251) young adults. L-AChR clusters (unc-29::tagRFP knock-in) localize at NMJs at dorsal and ventral SABs (delimited by filled arrowheads), at dorsal and ventral nerve cords (open arrowheads), and at the nerve ring (bracket). Multiple ectopic clusters are observed between the rows of dorsal and ventral synaptic SAB clusters in rsu-1(kr251). Right column, Magnifications of the boxed regions emphasizing the presence of ectopic L-AChR clusters in rsu-1(kr251) (arrows) compared with wild type. In this figure and all other figures, anterior is to the left and dorsal is up. Scale bars, 10 μm. C, Schematic domain structure of RSU-1. The position of the first residue of each LRR domains identified using LRR finder are indicated (Offord and Werling, 2013). Gray lines are regions with low complexity. D, Clustal Omega (EBI) alignment of the N terminus of RSU-1 orthologs from nematodes, fly, and vertebrates. The two first LRRs are boxed. Color code used to highlight residues is as follows: conserved residues in dark gray, residues with strongly similar properties (scoring >0.5) in medium gray, and residues with weakly similar properties (scoring ≤0.5) in light gray. Percentages of identity between full-length RSU-1 orthologs are presented in the table. Low complexity regions were included for evaluating percentages of identity.