Figure 2.

RSU-1 localizes in muscles at integrin adhesion complexes by interacting with UNC-97/PINCH. A, Top, An artificial operon containing the gfp sequence expressed under the control of an rsu-1 genomic fragment drives GFP expression in body wall, pharyngeal (px), and vulval muscles (vm). Scale bar, 50 μm. Bottom left, Zoom of the boxed region. A subset of neuron cell bodies (arrows) can be seen at higher magnification. Bottom right, GFP is also observed in the gonad distal tip cell (highlighted with white dotted circles). B, Confocal projections of one muscle cell of a fixed rsu-1(kr251) young adult expressing RSU-1-GFP translational reporter and immunolabeled with anti-GFP (top) and anti-PAT-3 (middle) antibodies. RSU-1 and PAT-3 colocalize to dense bodies (one is shown by an arrowhead), M-lines (one is shown by an arrow), and intercellular junctions (between thin arrowheads). C, RSU-1-GFP (left) and PAT-3 (right) distributions in young adults of different genetic backgrounds. RSU-1-GFP observations and anti-PAT-3 immunostainings were performed on different animals. Scale bars, 10 μm.