Figure 3.
RSU-1 is not essential for integrin adhesion complexes assembly. Actin filaments, DEB-1/vinculin, UNC-112/fermitin, and PAT-3/β-integrin distributions in wild-type and rsu-1(kr251) mutant adults. Actin, DEB-1, and PAT-3 were visualized using phalloidin–rhodamine staining and immunodetection with MH24 and MH25 antibodies, respectively. For UNC-112/fermitin, unc-112(st581) and rsu-1(kr251); unc-112(st581) expressing the raEx16[rol 6(su1006); unc 112(+)::GFP] rescuing transgene were observed. Red brackets delimit intercellular adhesion plaques that are less packed in rsu-1(kr251) compared with wild type. Scale bars, 10 μm.
